The effects of Alisma orientale S. and Zea mays L. water extract on the water uptke and urine flow was investigated in the intact frog. 86 frogs were divided into two groups : immersion and injection. Immersion-group was submerged in 100m1 of diluted solution containing Alisma orientale S. extract(0. 3%, lg%) and Zea mays L. exract (0. 3g%, lg%), and 100m1 of distilled water as a control.
Injection-group was injected Alisma orientale S. extract (20mg, 60mg)and Zea mays L. extract(40mg, 120mg) into the dorsal lymphatic sac, and submerged in 100m1 of distilled water. Body weight, urine flow and urinary concentration of chloride and osmolarity were measured for 1 hour interval during 3 hours under continuous oxygen supply at room temperature.
The results obtained summerized as follows :
1. Relationships between urine flow rate and body weight difference of pre-and-post urine emptying were statistically significant in all cases.
2. Urine flow rates were significantly increased(P<0.05) for first 1 hour in groups injected Alisma orientale S. extract and groups immersed in Zea mays L. extract.
3. Increase rates of body weight (rate of water uptake) were increased significantly (P<0. 05) for first 1 hour in groups injected Alisma orientale S. extract and groups immersed in Zea mays L. extract.
4. Urinary osmotic concentrations were inversely related to urine flow rate in all groups, and the excretion rates of osmolar particles were significantly increased(P<0. 05) when urine flow level were high. -
5. Urinary chloride concentration was increased for first 1 hour in the groups immersed in Alisma orientale S. extract.
In the above results, it suggest that increased urine formation was due to the increased water uptake across the frog skin in both groups injected Alisma orientale S. extract and immersed Zea mays L. extract, and their mechanism of action was different.
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